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protease inhibitor cocktail set iii  (Millipore)


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    Structured Review

    Millipore protease inhibitor cocktail set iii
    Protease Inhibitor Cocktail Set Iii, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protease+inhibitor+cocktail+set+iii/protease+inhibitor+cocktail+set+iii/pmc06120666__EMBJ___37___e99372___s001-59-28-33
    Average 90 stars, based on 1 article reviews
    protease inhibitor cocktail set iii - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Extracellular vesicles adhere to cells primarily by interactions of integrins and GM1 with laminin.
    Article Snippet: .. For western blotting, the pellet was suspended in RIPA buffer containing protease inhibitor cocktail set III (Millipore). .. The ExoSparkler Exosome Membrane Labeling Kit-Deep Red (Dojindo) was also utilized as a fluorescent probe.

    Protease Inhibitor:

    Article Title: Extracellular vesicles adhere to cells primarily by interactions of integrins and GM1 with laminin.
    Article Snippet: .. For western blotting, the pellet was suspended in RIPA buffer containing protease inhibitor cocktail set III (Millipore). .. The ExoSparkler Exosome Membrane Labeling Kit-Deep Red (Dojindo) was also utilized as a fluorescent probe.

    Article Title: Resveratrol’s Pro-Apoptotic Effects in Cancer Are Mediated Through the Interaction and Oligomerization of the Mitochondrial VDAC1
    Article Snippet: Annexin V-fluorescein isothiocyanate (FITC) was from Enzo Life Sciences (Lausanne, Switzerland). .. Protease inhibitor cocktail set III and digitonin was obtained from Calbiochem (Nottingham, UK). .. Fluo-4-AM, MitoSOX Red and dichlorofluorescein (DCF) were acquired from Thermo Fisher Scientific (Waltham, MA, USA).

    Article Title: Extracellular vesicles adhere to cells primarily by interactions of integrins and GM1 with laminin
    Article Snippet: .. The cells and sEVs were suspended in RIPA buffer containing protease inhibitor cocktail set III (Millipore). .. The protein concentrations of the samples were determined using a Thermo BCA protein assay kit (Thermo Fisher Scientific) and adjusted to 1 mg/ml for SDS-PAGE.

    Article Title: Small-molecule inhibitors reveal an indispensable scaffolding role of RIPK2 in NOD2 signaling APPENDIX
    Article Snippet: For expression, viruses were used to infect Sf9 cells grown in Insect-Xpress media (Lonza) to a density of 2x10 6 cells/mL. .. Cells were harvested after 48 hours by centrifugation and resuspended in binding buffer (50 mM HEPES pH 7.4, 500 mM NaCl, 5% glycerol, 5 mM Imidazole) supplemented with protease inhibitor cocktail set III (Calbiochem, Burlington, MA) at 1:1000 dilution. .. For expression, viruses were used to infect Sf9 cells grown in Insect-Xpress media (Lonza) to a density of 2x10 6 cells/mL.

    Article Title: Titin cleavage in living cardiomyocytes induces sarcomere disassembly but does not trigger cell proliferation
    Article Snippet: .. Cell lysates were obtained in 150 μ l of sample buffer (3% SDS, 50 mM TrisCl pH 6.8) supplemented with 50 mM N-ethylmaleimide (Sigma-Aldrich, E3876) and protease inhibitor Cocktail Set III (Millipore). ..

    Article Title: Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brain.
    Article Snippet: They were transfected with varying concentrations of plasmid (0, 0.1, 0.5, 1, 2, 5 μg) diluted in 200 μl Opti-MEM (1×; Gibco, 31985-062) with 3 μl Lipofectamine 2000 (Invitrogen, 52887). .. Twenty-four hours after transfection, cells were washed with ice-cold PBS and lysed with supplemented RIPA buffer (50 mM Tris, 150 mM NaCl, 1.0% (wt/vol) NP-40, 0.5% (wt/vol) sodium deoxycholate and 0.1% (wt/vol) SDS at a pH of 7.4) supplemented with Protease inhibitor cocktail set III (Calbiochem, 539134), cantharidin (500 nM in dimethylsulfoxide) and phosphatase inhibitor cocktail (16 mM Na2MO4, 16 mM NaF, 32 mM β-glycerophosphate, 4 mM Na3VO4 in dd.H2O). ..

    Article Title: Fatty acid synthase global inducible knockout does not alter brain fatty acid concentrations but attenuates cholesterol synthesis in the adult mouse.
    Article Snippet: Fatty acid (FA) de novo synthesis, also called de novo lipogenesis (DNL), has a central role in peripheral energy storage and provides structural components for lipid membranes.. However, less is known regarding its contribution to brain FA homeostasis.. DNL is catalyzed by fatty acid synthase (FAS), which is a multifunctional enzyme expressed in all mammalian tissues.

    Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
    Article Snippet: .. Cells (2 × 10 6 /sample) were lysed in 1%(v/v) Triton-X100 in 20 mM Tris–HCl (pH 8), 150 mM NaCl in the presence of Protease inhibitor Cocktail Set III (Calbiochem, Merck, Darmstadt, Germany) and the phosphatase inhibitor sodium orthovanadate (Sigma-Aldrich) for 5 min on ice. .. Protein extracts from post-nuclear supernatants were quantified using the Quantum protein assay kit (Euroclone) and denatured in 4x Bolt SDS sample buffer (Invitrogen, Waltham, Massachusetts, USA) supplemented with 10x Bolt sample reducing buffer (Invitrogen) for 5 min at 100 °C.

    Centrifugation:

    Article Title: Small-molecule inhibitors reveal an indispensable scaffolding role of RIPK2 in NOD2 signaling APPENDIX
    Article Snippet: For expression, viruses were used to infect Sf9 cells grown in Insect-Xpress media (Lonza) to a density of 2x10 6 cells/mL. .. Cells were harvested after 48 hours by centrifugation and resuspended in binding buffer (50 mM HEPES pH 7.4, 500 mM NaCl, 5% glycerol, 5 mM Imidazole) supplemented with protease inhibitor cocktail set III (Calbiochem, Burlington, MA) at 1:1000 dilution. .. For expression, viruses were used to infect Sf9 cells grown in Insect-Xpress media (Lonza) to a density of 2x10 6 cells/mL.

    Binding Assay:

    Article Title: Small-molecule inhibitors reveal an indispensable scaffolding role of RIPK2 in NOD2 signaling APPENDIX
    Article Snippet: For expression, viruses were used to infect Sf9 cells grown in Insect-Xpress media (Lonza) to a density of 2x10 6 cells/mL. .. Cells were harvested after 48 hours by centrifugation and resuspended in binding buffer (50 mM HEPES pH 7.4, 500 mM NaCl, 5% glycerol, 5 mM Imidazole) supplemented with protease inhibitor cocktail set III (Calbiochem, Burlington, MA) at 1:1000 dilution. .. For expression, viruses were used to infect Sf9 cells grown in Insect-Xpress media (Lonza) to a density of 2x10 6 cells/mL.

    Transfection:

    Article Title: Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brain.
    Article Snippet: They were transfected with varying concentrations of plasmid (0, 0.1, 0.5, 1, 2, 5 μg) diluted in 200 μl Opti-MEM (1×; Gibco, 31985-062) with 3 μl Lipofectamine 2000 (Invitrogen, 52887). .. Twenty-four hours after transfection, cells were washed with ice-cold PBS and lysed with supplemented RIPA buffer (50 mM Tris, 150 mM NaCl, 1.0% (wt/vol) NP-40, 0.5% (wt/vol) sodium deoxycholate and 0.1% (wt/vol) SDS at a pH of 7.4) supplemented with Protease inhibitor cocktail set III (Calbiochem, 539134), cantharidin (500 nM in dimethylsulfoxide) and phosphatase inhibitor cocktail (16 mM Na2MO4, 16 mM NaF, 32 mM β-glycerophosphate, 4 mM Na3VO4 in dd.H2O). ..

    Sonication:

    Article Title: Fatty acid synthase global inducible knockout does not alter brain fatty acid concentrations but attenuates cholesterol synthesis in the adult mouse.
    Article Snippet: Fatty acid (FA) de novo synthesis, also called de novo lipogenesis (DNL), has a central role in peripheral energy storage and provides structural components for lipid membranes.. However, less is known regarding its contribution to brain FA homeostasis.. DNL is catalyzed by fatty acid synthase (FAS), which is a multifunctional enzyme expressed in all mammalian tissues.



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    Entinostat effect on microRNA levels and microRNA-dependent regulation of human epidermal growth factor receptor 2 (HER2) expression. (A) Schematic illustration of miRNAs regulating HER2 and/or affected by HER2. (B) Alterations in miRNA expression levels in NCI-N87 cells after entinostat treatment for 48 hours with the indicated concentrations. (C) Time-dependent reduction of HER2 protein levels upon transfection of NCI-N87 cells with 10 nM of miR-205-3p. (D) Effect of anti-miR transfection on HER2 expression upon entinostat treatment. HER2 levels after transfection of control miRNA (ctrl) were compared with HER2 levels after transfection of specific <t>miR-205</t> <t>inhibitor</t> (205). For the protein analyses, HER2 levels were monitored 72 hours after miR (C) or anti-miR (D) transfection. Mean±standard error of mean of <t>three</t> independent experiments are given. a) p < 0.05.
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    Entinostat effect on microRNA levels and microRNA-dependent regulation of human epidermal growth factor receptor 2 (HER2) expression. (A) Schematic illustration of miRNAs regulating HER2 and/or affected by HER2. (B) Alterations in miRNA expression levels in NCI-N87 cells after entinostat treatment for 48 hours with the indicated concentrations. (C) Time-dependent reduction of HER2 protein levels upon transfection of NCI-N87 cells with 10 nM of miR-205-3p. (D) Effect of anti-miR transfection on HER2 expression upon entinostat treatment. HER2 levels after transfection of control miRNA (ctrl) were compared with HER2 levels after transfection of specific <t>miR-205</t> <t>inhibitor</t> (205). For the protein analyses, HER2 levels were monitored 72 hours after miR (C) or anti-miR (D) transfection. Mean±standard error of mean of <t>three</t> independent experiments are given. a) p < 0.05.
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    Image Search Results


    Entinostat effect on microRNA levels and microRNA-dependent regulation of human epidermal growth factor receptor 2 (HER2) expression. (A) Schematic illustration of miRNAs regulating HER2 and/or affected by HER2. (B) Alterations in miRNA expression levels in NCI-N87 cells after entinostat treatment for 48 hours with the indicated concentrations. (C) Time-dependent reduction of HER2 protein levels upon transfection of NCI-N87 cells with 10 nM of miR-205-3p. (D) Effect of anti-miR transfection on HER2 expression upon entinostat treatment. HER2 levels after transfection of control miRNA (ctrl) were compared with HER2 levels after transfection of specific miR-205 inhibitor (205). For the protein analyses, HER2 levels were monitored 72 hours after miR (C) or anti-miR (D) transfection. Mean±standard error of mean of three independent experiments are given. a) p < 0.05.

    Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

    Article Title: The Histone Deacetylase Inhibitor Entinostat Mediates HER2 Downregulation in Gastric Cancer, Providing the Basis for Its Particular Efficacy in HER2 -Amplified Tumors and in Combination Therapies

    doi: 10.4143/crt.2024.546

    Figure Lengend Snippet: Entinostat effect on microRNA levels and microRNA-dependent regulation of human epidermal growth factor receptor 2 (HER2) expression. (A) Schematic illustration of miRNAs regulating HER2 and/or affected by HER2. (B) Alterations in miRNA expression levels in NCI-N87 cells after entinostat treatment for 48 hours with the indicated concentrations. (C) Time-dependent reduction of HER2 protein levels upon transfection of NCI-N87 cells with 10 nM of miR-205-3p. (D) Effect of anti-miR transfection on HER2 expression upon entinostat treatment. HER2 levels after transfection of control miRNA (ctrl) were compared with HER2 levels after transfection of specific miR-205 inhibitor (205). For the protein analyses, HER2 levels were monitored 72 hours after miR (C) or anti-miR (D) transfection. Mean±standard error of mean of three independent experiments are given. a) p < 0.05.

    Article Snippet: The next day, cells were treated for 72 hours, prior to harvesting and lysis in 20-50 μL RIPA buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate) containing protease inhibitor cocktail set III, EDTA-free (1:500, Merck Darmstadt).

    Techniques: Expressing, Transfection, Control

    Effect of combination therapy with entinostat plus selective human epidermal growth factor receptor 2 (HER2) inhibitor CP724714. (A) Analysis of HER2 protein expression in MKN-74 cells treated for 72 hours with vehicle dimethyl sulfoxide (DMSO, D), 1.5 μM entinostat (E), 2.0 μM CP-724714 (CP), or the combination of both (E+CP). Densitometric quantifications of three independent experiments (mean±standard error of mean [SEM]) and one representative western blot are shown. (B) Effect of entinostat, CP724714, or the combination of both on proliferation of MKN-74 cells in comparison to vehicle-treated cells as determined by Cell Counting Kit 8 (CCK8) assay (mean±SEM, n=3). (C) Effect on colony formation of MKN-74 after treatment with entinostat, CP, or entinostat+CP. Scale bars=500 µm. (D) Combinatory effect on cell death, analysis was conducted using flow cytometry-based distribution of propidium iodide (PI) and annexin-V–stained cells. (E) Combinatory effect on cell cycle, analysis was conducted using flow cytometry–based cell cycle distribution of PI-stained cells. Quantitation of three independent experiments (mean±SEM) are shown in (D) and (E). a) p < 0.05, b) p < 0.03, c) p < 0.01.

    Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

    Article Title: The Histone Deacetylase Inhibitor Entinostat Mediates HER2 Downregulation in Gastric Cancer, Providing the Basis for Its Particular Efficacy in HER2 -Amplified Tumors and in Combination Therapies

    doi: 10.4143/crt.2024.546

    Figure Lengend Snippet: Effect of combination therapy with entinostat plus selective human epidermal growth factor receptor 2 (HER2) inhibitor CP724714. (A) Analysis of HER2 protein expression in MKN-74 cells treated for 72 hours with vehicle dimethyl sulfoxide (DMSO, D), 1.5 μM entinostat (E), 2.0 μM CP-724714 (CP), or the combination of both (E+CP). Densitometric quantifications of three independent experiments (mean±standard error of mean [SEM]) and one representative western blot are shown. (B) Effect of entinostat, CP724714, or the combination of both on proliferation of MKN-74 cells in comparison to vehicle-treated cells as determined by Cell Counting Kit 8 (CCK8) assay (mean±SEM, n=3). (C) Effect on colony formation of MKN-74 after treatment with entinostat, CP, or entinostat+CP. Scale bars=500 µm. (D) Combinatory effect on cell death, analysis was conducted using flow cytometry-based distribution of propidium iodide (PI) and annexin-V–stained cells. (E) Combinatory effect on cell cycle, analysis was conducted using flow cytometry–based cell cycle distribution of PI-stained cells. Quantitation of three independent experiments (mean±SEM) are shown in (D) and (E). a) p < 0.05, b) p < 0.03, c) p < 0.01.

    Article Snippet: The next day, cells were treated for 72 hours, prior to harvesting and lysis in 20-50 μL RIPA buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate) containing protease inhibitor cocktail set III, EDTA-free (1:500, Merck Darmstadt).

    Techniques: Expressing, Western Blot, Comparison, Cell Counting, CCK-8 Assay, Flow Cytometry, Staining, Cell Cycle Assay, Quantitation Assay